Coding

Part:BBa_K2635005

Designed by: Hsuan Cheng, Ching-Lin Kao, Yi-Chien Chuang   Group: iGEM18_NTHU_Formosa   (2018-10-03)


TEV protease C-terminal

TEV proteases are the 27 kDa catalytic domains of the NIa (Nuclear Inclusion a) protein encoded by Tobacco Etch Virus (TEV), where TEV proteases cut polyproteins into single proteins during biogenesis. In our design, TEV protease is split into N-terminal and C- terminal fragments. When split GBP is brought together by the presence of GFP, the two TEV protease fragments will combine and cleave TEV cutting sites. After the cleavage, tTA is freed and translocated in to the nucleus and bind to TRE3G, the promoter driving output emission signal.[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 165
    Illegal SapI.rc site found at 475


References

  1. ↑ Baeumler, T. A., Ahmed, A. A., &Fulga, T. A. (2017). Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Reports, 20(11), 2639–2653. https://doi.org/10.1016/j.celrep.2017.08.044
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